\(\textit{In vitro}\) regeneration of \(\textit{Renanthera imschootiana}\) Rolfe from protocorm-like body

Renanthera imschootiana Rolfe is an extremely rare and endangered tropical epiphytic orchid. Studies on in vitro culture of R. were conducted order to conserve increase the genetic pool this wild orchid species. In study, effect plant growth regulators (BA NAA), ripe bananas combination with potato, humic acid, silver nanoparticles (AgNPs) investigated find a suitable condition for regeneration from protocorm-like body (PLB). The results showed that after 45 days culture, MS medium supplemented 100 g banana/liter potato/liter was regenerating shoots (PLB) (17.56 shoots/PLBs 100.00% shoot-regenerating PLBs); 2.0 mg/L BA, 0.3 NAA 4 ppm AgNPs most 60 (10.00 leaves/shoot shoot length 4.22 cm). transferred half-strength acid investigate root formation imschootiana. After best rooting obtained at 6 (8.33 roots/shoot; 4.00 cm formation). findings suggest micropropagation provides useful alternative tool conservation